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Neutralizing antibodies can initiate genome release from human enterovirus 71.

Plevka P, Lim PY, Perera R, Cardosa J, Suksatu A, Kuhn RJ, Rossmann MG
Proc Natl Acad Sci U S A 111 2134-9 (2014)
Related entries: 4c0u, 4c10

Cited: 43 times
EuropePMC logo PMID: 24469789

Abstract

Antibodies were prepared by immunizing mice with empty, immature particles of human enterovirus 71 (EV71), a picornavirus that causes severe neurological disease in young children. The capsid structure of these empty particles is different from that of the mature virus and is similar to "A" particles encountered when picornaviruses recognize a potential host cell before genome release. The monoclonal antibody E18, generated by this immunization, induced a conformational change when incubated at temperatures between 4 °C and 37 °C with mature virus, transforming infectious virions into A particles. The resultant loss of genome that was observed by cryo-EM and a fluorescent SYBR Green dye assay inactivated the virus, establishing the mechanism by which the virus is inactivated and demonstrating that the E18 antibody has potential as an anti-EV71 therapy. The antibody-mediated virus neutralization by the induction of genome release has not been previously demonstrated. Furthermore, the present results indicate that antibodies with genome-release activity could also be produced for other picornaviruses by immunization with immature particles.

Articles - 4c0y mentioned but not cited (1)

  1. Neutralizing antibodies can initiate genome release from human enterovirus 71. Plevka P, Lim PY, Perera R, Cardosa J, Suksatu A, Kuhn RJ, Rossmann MG. Proc Natl Acad Sci U S A 111 2134-2139 (2014)


Reviews citing this publication (9)

  1. Update on enterovirus 71 infection. Huang PN, Shih SR. Curr Opin Virol 5 98-104 (2014)
  2. Immunopathogenesis and Virus-Host Interactions of Enterovirus 71 in Patients with Hand, Foot and Mouth Disease. Cox JA, Hiscox JA, Solomon T, Ooi MH, Ng LFP. Front Microbiol 8 2249 (2017)
  3. Viral entry pathways: the example of common cold viruses. Blaas D. Wien Med Wochenschr 166 211-226 (2016)
  4. Recent development of enterovirus A vaccine candidates for the prevention of hand, foot, and mouth disease. Fang CY, Liu CC. Expert Rev Vaccines 17 819-831 (2018)
  5. Evolutionary and Structural Overview of Human Picornavirus Capsid Antibody Evasion. Cifuente JO, Moratorio G. Front Cell Infect Microbiol 9 283 (2019)
  6. Cryo-EM Studies of Virus-Antibody Immune Complexes. Li N, Li Z, Fu Y, Cao S. Virol Sin 35 1-13 (2020)
  7. From Monovalent to Multivalent Vaccines, the Exploration for Potential Preventive Strategies Against Hand, Foot, and Mouth Disease (HFMD). He X, Zhang M, Zhao C, Zheng P, Zhang X, Xu J. Virol Sin 36 167-175 (2021)
  8. Functional assessment and structural basis of antibody binding to human papillomavirus capsid. Zhang X, Li S, Modis Y, Li Z, Zhang J, Xia N, Zhao Q. Rev Med Virol 26 115-128 (2016)
  9. Mechanisms of Rhinovirus Neutralisation by Antibodies. Touabi L, Aflatouni F, McLean GR. Viruses 13 360 (2021)

Articles citing this publication (33)



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