4c4w

X-ray diffraction
2.95Å resolution

Structure of a rare, non-standard sequence k-turn bound by L7Ae protein

Released:
Primary publication:
Structure of a rare non-standard sequence k-turn bound by L7Ae protein.
OpenAccess logo Nucleic Acids Res 42 4734-40 (2014)
PMID: 24482444

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-128131 (preferred)
Entry contents:
2 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (3 distinct):
U1 small nuclear ribonucleoprotein A Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 102 amino acids
Theoretical weight: 11.74 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09012 (Residues: 1-102; Coverage: 36%)
Gene name: SNRPA
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
Large ribosomal subunit protein eL8 Chains: C, G
Molecule details ›
Chains: C, G
Length: 123 amino acids
Theoretical weight: 13.52 KDa
Source organism: Archaeoglobus fulgidus DSM 4304
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29494 (Residues: 2-119; Coverage: 99%)
Gene names: AF_0764, rpl7ae
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
TSKT-23 Chains: D, H
Molecule details ›
Chains: D, H
Length: 35 nucleotides
Theoretical weight: 11.2 KDa

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2221
Unit cell:
a: 135.892Å b: 155.545Å c: 146.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.211
Expression system: Escherichia coli BL21(DE3)