Function and Biology Details
Reactions catalysed:
S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
A 5'-triphospho-[mRNA] + H(2)O = a 5'-diphospho-[mRNA] + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- S-adenosyl-L-methionine-dependent methyltransferase superfamily
- mRNA (guanine-N(7))-methyltransferase domain
- Poxvirus mRNA capping enzyme, small subunit superfamily
- Poxvirus mRNA capping enzyme, small subunit
- mRNA cap guanine-N7 methyltransferase
- mRNA-capping enzyme catalytic subunit, OB fold domain superfamily
- mRNA-capping enzyme catalytic subunit, nucleotidyltransferase domain superfamily
- mRNA capping enzyme catalytic subunit, RNA triphosphatase domain, virus
1 more domain
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
mRNA-capping enzyme (preferred)
PDBe Complex ID:
PDB-CPX-137587 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA-capping enzyme catalytic subunit
Molecule details ›
Chains: A, D
Length: 844 amino acids
Theoretical weight: 96.86 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains:
Length: 844 amino acids
Theoretical weight: 96.86 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P04298 (Residues: 1-844; Coverage: 100%)
Sequence domains:
- mRNA capping enzyme catalytic subunit, RNA triphosphatase domain
- mRNA capping enzyme catalytic subunit, GTase, NTPase domain
- mRNA-capping enzyme catalytic subunit, GTase, OB fold
- mRNA (guanine-N(7))-methyltransferase domain
mRNA-capping enzyme regulatory subunit OPG124
Molecule details ›
Chains: B, E
Length: 287 amino acids
Theoretical weight: 33.4 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Poxvirus mRNA capping enzyme, small subunit
Structure domains: Poxvirus mRNA capping enzyme, small subunit
Length: 287 amino acids
Theoretical weight: 33.4 KDa
Source organism: Vaccinia virus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P04318 (Residues: 1-287; Coverage: 100%)
Sequence domains: Poxvirus mRNA capping enzyme, small subunit
Structure domains: Poxvirus mRNA capping enzyme, small subunit
