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A soluble mutant of the transmembrane receptor Af1503 features strong changes in coiled-coil periodicity.

Hartmann MD, Dunin-Horkawicz S, Hulko M, Martin J, Coles M, Lupas AN
J Struct Biol 186 357-66 (2014)
Cited: 13 times
EuropePMC logo PMID: 24568954

Abstract

Structures of full-length, membrane-bound proteins are essential for understanding transmembrane signaling mechanisms. However, in prokaryotic receptors no such structure has been reported, despite active research for many years. Here we present results of an alternative strategy, whereby a transmembrane receptor is made soluble by selective mutations to the membrane-spanning region, chosen by analysis of helix geometry in the transmembrane regions of chemotaxis receptors. We thus converted the receptor Af1503 from Archaeoglobus fulgidus to a soluble form by deleting transmembrane helix 1 and mutating the surface residues of transmembrane helix 2 to hydrophilic amino acids. Crystallization of this protein resulted in the structure of a tetrameric proteolytic fragment representing the modified transmembrane helices plus the cytoplasmic HAMP domain, a ubiquitous domain of prokaryotic signal transducers. The protein forms a tetramer via native parallel dimerization of the HAMP domain and non-native antiparallel dimerization of the modified transmembrane helices. The latter results in a four-helical coiled coil, characterized by unusually large changes in helix periodicity. The structure offers the first view of the junction between the transmembrane region and HAMP and explains the conservation of a key sequence motif in HAMP domains.

Articles - 4cq4 mentioned but not cited (4)

  1. A library of coiled-coil domains: from regular bundles to peculiar twists. Szczepaniak K, Bukala A, da Silva Neto AM, Ludwiczak J, Dunin-Horkawicz S. Bioinformatics 36 5368-5376 (2021)
  2. ZapG (YhcB/DUF1043), a novel cell division protein in gamma-proteobacteria linking the Z-ring to septal peptidoglycan synthesis. Mehla J, Liechti G, Morgenstein RM, Caufield JH, Hosseinnia A, Gagarinova A, Phanse S, Goodacre N, Brockett M, Sakhawalkar N, Babu M, Xiao R, Montelione GT, Vorobiev S, den Blaauwen T, Hunt JF, Uetz P. J Biol Chem 296 100700 (2021)
  3. Assessment of domain interactions in the fourteenth round of the Critical Assessment of Structure Prediction (CASP14). Schaeffer RD, Kinch L, Kryshtafovych A, Grishin NV. Proteins 89 1700-1710 (2021)
  4. AlphaFold2 captures the conformational landscape of the HAMP signaling domain. Winski A, Ludwiczak J, Orlowska M, Madaj R, Kaminski K, Dunin-Horkawicz S. Protein Sci 33 e4846 (2024)


Reviews citing this publication (2)

  1. C-di-GMP Synthesis: Structural Aspects of Evolution, Catalysis and Regulation. Schirmer T. J Mol Biol 428 3683-3701 (2016)
  2. Bacterial chemoreceptors and chemoeffectors. Bi S, Lai L. Cell Mol Life Sci 72 691-708 (2015)

Articles citing this publication (7)

  1. Mechanism of transmembrane signaling by sensor histidine kinases. Gushchin I, Melnikov I, Polovinkin V, Ishchenko A, Yuzhakova A, Yuzhakova A, Buslaev P, Bourenkov G, Grudinin S, Round E, Balandin T, Borshchevskiy V, Willbold D, Leonard G, Büldt G, Popov A, Gordeliy V. Science 356 eaah6345 (2017)
  2. Axial helix rotation as a mechanism for signal regulation inferred from the crystallographic analysis of the E. coli serine chemoreceptor. Ferris HU, Zeth K, Hulko M, Dunin-Horkawicz S, Lupas AN. J Struct Biol 186 349-356 (2014)
  3. Computational models in the service of X-ray and cryo-electron microscopy structure determination. Kryshtafovych A, Moult J, Albrecht R, Chang GA, Chao K, Fraser A, Greenfield J, Hartmann MD, Herzberg O, Josts I, Leiman PG, Linden SB, Lupas AN, Nelson DC, Rees SD, Shang X, Sokolova ML, Tidow H, AlphaFold2 team. Proteins 89 1633-1646 (2021)
  4. STAC--A New Domain Associated with Transmembrane Solute Transport and Two-Component Signal Transduction Systems. Korycinski M, Albrecht R, Ursinus A, Hartmann MD, Coles M, Martin J, Dunin-Horkawicz S, Lupas AN. J Mol Biol 427 3327-3339 (2015)
  5. α/β coiled coils. Hartmann MD, Mendler CT, Bassler J, Karamichali I, Ridderbusch O, Lupas AN, Hernandez Alvarez B. Elife 5 e11861 (2016)
  6. New Insights on Signal Propagation by Sensory Rhodopsin II/Transducer Complex. Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Remeeva A, Polovinkin V, Utrobin P, Balandin T, Engelhard M, Büldt G, Gordeliy V. Sci Rep 7 41811 (2017)
  7. Stability and Conformation of a Chemoreceptor HAMP Domain Chimera Correlates with Signaling Properties. Sukomon N, Widom J, Borbat PP, Freed JH, Crane BR. Biophys J 112 1383-1395 (2017)


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