› 4cw2

X-ray diffraction
1.32Å resolution

Crystal structure of cofactor-free urate oxidase in complex with the 5-peroxo derivative of 9-metyl uric acid (X-ray dose, 2.5 kGy)

Released:
DOI: 10.2210/pdb4cw2/pdb
PDB entry 4cw2 coloured by chain, front view.
PDB entry 4cw2 coloured by chain, side view.
PDB entry 4cw2 coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Direct evidence for a peroxide intermediate and a reactive enzyme-substrate-dioxygen configuration in a cofactor-free oxidase.
Bui S, von Stetten D, Jambrina PG, Prangé T, Colloc'h N, de Sanctis D, Royant A, Rosta E, Steiner RA
OpenAccess logo Angew Chem Int Ed Engl 53 13710-4 (2014)
PMID: 25314114

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 301 amino acids
Theoretical weight: 34.2 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

2 bound ligands:
Ligand XDS 1 x XDS
Ligand MPD 1 x MPD
1 modified residue:
Ligand SAC 1 x SAC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I222
Unit cell:
a: 79.506Å b: 95.132Å c: 104.31Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.112 0.11 0.137
Expression system: Saccharomyces cerevisiae

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Citations

3 review citations

Radiation damage to macromolecules: kill or cure?
Garman et al. (2015)
2 more

1 mention without citation

Rapid and efficient room-temperature serial synchrotron crystallography using the CFEL TapeDrive.
Zielinski et al. (2022)