› 4d1d

X-ray diffraction
3.7Å resolution

STRUCTURE OF MHP1, A NUCLEOBASE-CATION-SYMPORT-1 FAMILY TRANSPORTER with the inhibitor 5-(2-naphthylmethyl)-L-hydantoin.

Released:
DOI: 10.2210/pdb4d1d/pdb
PDB entry 4d1d coloured by chain, front view.
PDB entry 4d1d coloured by chain, side view.
PDB entry 4d1d coloured by chain, top view.
Source organism: Microbacterium liquefaciens
Primary publication:
Molecular mechanism of ligand recognition by membrane transport protein, Mhp1.
Simmons KJ, Jackson SM, Brueckner F, Patching SG, Beckstein O, Ivanova E, Geng T, Weyand S, Drew D, Lanigan J, Sharples DJ, Sansom MS, Iwata S, Fishwick CW, Johnson AP, Cameron AD, Henderson PJ
OpenAccess logo EMBO J 33 1831-44 (2014)
PMID: 24952894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112805 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hydantoin permease Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 54.02 KDa
Source organism: Microbacterium liquefaciens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: D6R8X8 (Residues: 1-487; Coverage: 100%)
Gene name: hyuP
Sequence domains: Permease for cytosine/purines, uracil, thiamine, allantoin
Structure domains: Hydantoin permease

Ligands and Environments

3 bound ligands:
Ligand 5NL 1 x 5NL
Ligand 5ND 1 x 5ND
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 87.6Å b: 106.9Å c: 106.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.256 0.308
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Shared Molecular Mechanisms of Membrane Transporters.
Drew et al. (2016)
8 more

1 mention without citation

Molecular mechanism of ligand recognition by membrane transport protein, Mhp1.
Simmons et al. (2014)