› 4d4p

X-ray diffraction
3Å resolution

Crystal Structure of the Kti11 Kti13 heterodimer Spacegroup P65

Released:
DOI: 10.2210/pdb4d4p/pdb
PDB entry 4d4p coloured by chain, front view.
PDB entry 4d4p coloured by chain, side view.
PDB entry 4d4p coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Structure of the Kti11/Kti13 heterodimer and its double role in modifications of tRNA and eukaryotic elongation factor 2.
Glatt S, Zabel R, Vonkova I, Kumar A, Netz DJ, Pierik AJ, Rybin V, Lill R, Gavin AC, Balbach J, Breunig KD, Müller CW
Structure 23 149-160 (2015)
PMID: 25543256

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152000 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diphthamide biosynthesis protein 3; Protein KTI13 Chains: A, B, C, E, G, H
Molecule details ›
Chains: A, B, C, E, G, H
Length: 427 amino acids
Theoretical weight: 46.7 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P31386 (Residues: 1-333; Coverage: 100%)
  • Canonical: Q3E840 (Residues: 1-82; Coverage: 100%)
Gene names: ATS1, DPH3, FUN28, KTI11, KTI13, YAL006, YAL020C, YBL071W-A
Sequence domains:
Structure domains: DPH Zinc finger

Ligands and Environments

2 bound ligands:
Ligand FE 4 x FE
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: P65
Unit cell:
a: 152.06Å b: 152.06Å c: 203.85Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.208 0.234
Expression system: Escherichia coli BL21

Quick links

Citations

7 review citations

RNA modifications and cancer.
Haruehanroengra et al. (2020)
6 more

1 mention without citation

Degenerated Hole Doping and Ultra-Low Lattice Thermal Conductivity in Polycrystalline SnSe by Nonequilibrium Isovalent Te Substitution.
He et al. (2022)