› 4d7q

X-ray diffraction
2Å resolution

Crystal structure of a chimeric protein with the Sec7 domain of Legionella pneumophila RalF and the capping domain of Rickettsia prowazekii RalF

Released:
DOI: 10.2210/pdb4d7q/pdb
PDB entry 4d7q coloured by chain, front view.
PDB entry 4d7q coloured by chain, side view.
PDB entry 4d7q coloured by chain, top view.
Source organisms:
Primary publication:
On the use of Legionella/Rickettsia chimeras to investigate the structure and regulation of Rickettsia effector RalF.
Folly-Klan M, Sancerne B, Alix E, Roy CR, Cherfils J, Campanacci V
J Struct Biol 189 98-104 (2015)
PMID: 25498244

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186038 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SEC7 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 40.52 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8RT31 (Residues: 2-197; Coverage: 52%)
  • Canonical: Q9ZDF5 (Residues: 200-340; Coverage: 31%)
Gene names: JBJ86_14515, RP374, ralF
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand EPE 1 x EPE
Ligand NO3 1 x NO3
Ligand GOL 4 x GOL
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 40.31Å b: 77.44Å c: 119.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Allosteric regulation of Arf GTPases and their GEFs at the membrane interface.
Nawrotek et al. (2016)
3 more