› 4d8f

X-ray diffraction
2.2Å resolution

Chlamydia trachomatis NrdB with a Mn/Fe cofactor (procedure 1 - high Mn)

Released:
DOI: 10.2210/pdb4d8f/pdb
PDB entry 4d8f coloured by chain, front view.
PDB entry 4d8f coloured by chain, side view.
PDB entry 4d8f coloured by chain, top view.
Source organism: Chlamydia trachomatis
Primary publication:
Evidence that the β subunit of Chlamydia trachomatis ribonucleotide reductase is active with the manganese ion of its manganese(IV)/iron(III) cofactor in site 1.
Dassama LM, Boal AK, Krebs C, Rosenzweig AC, Bollinger JM
J Am Chem Soc 134 2520-3 (2012)
PMID: 22242660

Function and Biology Details

Reaction catalysed: 
2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131419 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonucleoside-diphosphate reductase subunit beta Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 366 amino acids
Theoretical weight: 42.73 KDa
Source organism: Chlamydia trachomatis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O84835 (Residues: 1-346; Coverage: 100%)
Gene names: CT_828, nrdB
Sequence domains: Ribonucleotide reductase, small chain
Structure domains: Ribonucleotide Reductase, subunit A

Ligands and Environments

3 bound ligands:
Ligand FE 4 x FE
Ligand MN 4 x MN
Ligand ACY 4 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 75.221Å b: 97.998Å c: 100.291Å
α: 90° β: 97.79° γ: 90°
R-values:
R R work R free
0.188 0.186 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Ribonucleotide reductases: essential enzymes for bacterial life.
Torrents E. (2014)
4 more

3 mentions without citation

Evidence that the β subunit of Chlamydia trachomatis ribonucleotide reductase is active with the manganese ion of its manganese(IV)/iron(III) cofactor in site 1.
Dassama et al. (2012)
2 more