› 4dc2

X-ray diffraction
2.4Å resolution

Structure of PKC in Complex with a Substrate Peptide from Par-3

Released:
DOI: 10.2210/pdb4dc2/pdb
PDB entry 4dc2 coloured by chain, front view.
PDB entry 4dc2 coloured by chain, side view.
PDB entry 4dc2 coloured by chain, top view.
Source organisms:
Primary publication:
Substrate recognition mechanism of atypical protein kinase Cs revealed by the structure of PKCĪ¹ in complex with a substrate peptide from Par-3.
Wang C, Shang Y, Yu J, Zhang M
Structure 20 791-801 (2012)
PMID: 22579248

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-563111 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein kinase C iota type Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 45.7 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q62074 (Residues: 231-595; Coverage: 61%)
Gene names: Pkcl, Prkci
Sequence domains:
Structure domains:
Partitioning defective 3 homolog Chain: Z
Molecule details ›
Chain: Z
Length: 28 amino acids
Theoretical weight: 3.21 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: Q9Z340 (Residues: 813-840; Coverage: 2%)
Gene names: Par3, Pard3

Ligands and Environments

1 bound ligand:
Ligand ADE 1 x ADE
1 modified residue:
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 101.456Å b: 54.9Å c: 82.489Å
α: 90° β: 115.18° γ: 90°
R-values:
R R work R free
0.176 0.172 0.241
Expression systems:
  • Spodoptera frugiperda
  • Not provided

Quick links

Citations

9 review citations

Mechanistic basis of MAGUK-organized complexes in synaptic development and signalling.
Zhu et al. (2016)
8 more

12 mentions without citation

Homing in: Mechanisms of Substrate Targeting by Protein Kinases.
Miller et al. (2018)
11 more