› 4dh7

X-ray diffraction
1.8Å resolution

Low temperature X-ray structure of cAMP dependent Protein Kinase A catalytic subunit with high Mg2+, AMP-PNP and IP20'

Released:
DOI: 10.2210/pdb4dh7/pdb
PDB entry 4dh7 coloured by chain, front view.
PDB entry 4dh7 coloured by chain, side view.
PDB entry 4dh7 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Low- and room-temperature X-ray structures of protein kinase A ternary complexes shed new light on its activity.
Kovalevsky AY, Johnson H, Hanson BL, Waltman MJ, Fisher SZ, Taylor S, Langan P
Acta Crystallogr D Biol Crystallogr 68 854-60 (2012)
PMID: 22751671

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138546 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
cAMP-dependent protein kinase catalytic subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P05132 (Residues: 2-351; Coverage: 100%)
Gene names: Pkaca, Prkaca
Sequence domains: Protein kinase domain
Structure domains:
cAMP-dependent protein kinase inhibitor alpha Chain: I
Molecule details ›
Chain: I
Length: 20 amino acids
Theoretical weight: 2.23 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: P63248 (Residues: 6-25; Coverage: 26%)
Gene name: Pkia

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ANP 1 x ANP
2 modified residues:
Ligand SEP 2 x SEP
Ligand TPO 1 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 57.535Å b: 79.651Å c: 98.011Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.179 0.179 0.194
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

13 citation in other articles

Divalent Metal Ions Mg²⁺ and Ca²⁺ Have Distinct Effects on Protein Kinase A Activity and Regulation.
Knape et al. (2015)
12 more

1 mention without citation

Low- and room-temperature X-ray structures of protein kinase A ternary complexes shed new light on its activity.
Kovalevsky et al. (2012)