› 4dne

X-ray diffraction
1.88Å resolution

Crystal structure of a triple-mutant of streptavidin in complex with desthiobiotin

Released:
DOI: 10.2210/pdb4dne/pdb
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PDB entry 4dne coloured by chain, front view.
PDB entry 4dne coloured by chain, side view.
PDB entry 4dne coloured by chain, top view.
Source organism: Streptomyces avidinii
Primary publication:
Contamination from an affinity column: an encounter with a new villain in the world of membrane-protein crystallization.
Panwar P, Deniaud A, Pebay-Peyroula E
Acta Crystallogr D Biol Crystallogr 68 1272-7 (2012)
PMID: 22993081

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149694 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Streptavidin Chains: A, B
Molecule details ›
Chains: A, B
Length: 183 amino acids
Theoretical weight: 18.89 KDa
Source organism: Streptomyces avidinii
Expression system: Escherichia coli
UniProt:
  • Canonical: P22629 (Residues: 1-183; Coverage: 100%)
Sequence domains: Avidin family
Structure domains: Avidin-like

Ligands and Environments

2 bound ligands:
Ligand DTB 2 x DTB
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I222
Unit cell:
a: 46.72Å b: 93.782Å c: 104.413Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.181 0.219
Expression system: Escherichia coli

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Citations

1 review citation

Impact of residual impurities and contaminants on protein stability.
Wang et al. (2014)
2 other articles

2 mentions without citation

Molecular replacement then and now.
Scapin G. (2013)
1 more

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