› 4dsh

X-ray diffraction
2.25Å resolution

Crystal structure of reduced UDP-Galactopyranose mutase

Released:
DOI: 10.2210/pdb4dsh/pdb
PDB entry 4dsh coloured by chain, front view.
PDB entry 4dsh coloured by chain, side view.
PDB entry 4dsh coloured by chain, top view.
Source organism: Trypanosoma cruzi
Primary publication:
Crystal structures of Trypanosoma cruzi UDP-galactopyranose mutase implicate flexibility of the histidine loop in enzyme activation.
Dhatwalia R, Singh H, Oppenheimer M, Sobrado P, Tanner JJ
Biochemistry 51 4968-79 (2012)
PMID: 22646091

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amine oxidase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 484 amino acids
Theoretical weight: 55.23 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4E1W2 (Residues: 1-480; Coverage: 100%)
Gene name: Tc00.1047053507993.160
Sequence domains: Flavin containing amine oxidoreductase
Structure domains: FAD/NAD(P)-binding domain

Ligands and Environments


Cofactor: Ligand FDA 2 x FDA
3 bound ligands:
Ligand UDP 2 x UDP
Ligand SO4 4 x SO4
Ligand EPE 1 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6522
Unit cell:
a: 143.84Å b: 143.84Å c: 354.389Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.18 0.21
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Noncanonical reactions of flavoenzymes.
Sobrado P. (2012)
2 more

8 mentions without citation

Copaifera of the Neotropics: A Review of the Phytochemistry and Pharmacology.
da Trindade et al. (2018)
7 more