› 4dvd

X-ray diffraction
3Å resolution

Crystal structure of the disulphide linked knotted homodimer of Psu

Released:
DOI: 10.2210/pdb4dvd/pdb
PDB entry 4dvd coloured by chain, front view.
PDB entry 4dvd coloured by chain, side view.
PDB entry 4dvd coloured by chain, top view.
Source organism: Enterobacteria phage P4
Primary publication:
The first structure of polarity suppression protein, Psu from enterobacteria phage P4, reveals a novel fold and a knotted dimer.
Banerjee R, Nath S, Ranjan A, Khamrui S, Pani B, Sen R, Sen U
J Biol Chem 287 44667-75 (2012)
PMID: 23150672

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138687 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polarity suppression protein Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.36 KDa
Source organism: Enterobacteria phage P4
Expression system: Escherichia coli
UniProt:
  • Canonical: P05460 (Residues: 1-190; Coverage: 100%)
Gene name: psu
Sequence domains: Phage polarity suppression protein (Psu)
Structure domains: Phage polarity suppression protein monomer

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR591
Spacegroup: I422
Unit cell:
a: 149.61Å b: 149.61Å c: 62.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.231 0.272
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Rho Protein: Roles and Mechanisms.
Mitra et al. (2017)
2 more

2 mentions without citation

The first structure of polarity suppression protein, Psu from enterobacteria phage P4, reveals a novel fold and a knotted dimer.
Banerjee et al. (2012)
1 more