› 4e4f

X-ray diffraction
2Å resolution

Crystal structure of enolase PC1_0802 (TARGET EFI-502240) from Pectobacterium carotovorum subsp. carotovorum PC1

Released:
DOI: 10.2210/pdb4e4f/pdb
PDB entry 4e4f coloured by chain, front view.
PDB entry 4e4f coloured by chain, side view.
PDB entry 4e4f coloured by chain, top view.
Source organism: Pectobacterium carotovorum subsp. carotovorum PC1
Entry authors: Patskovsky Y, Toro R, Bhosle R, Hillerich B, Seidel RD, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Zencheck WD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Reaction catalysed: 
D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111559 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactonate dehydratase family member PC1_0802 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 426 amino acids
Theoretical weight: 48.21 KDa
Source organism: Pectobacterium carotovorum subsp. carotovorum PC1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6D9S0 (Residues: 1-404; Coverage: 100%)
Gene name: PC1_0802
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 12 x GOL
Ligand CL 2 x CL
Ligand MG 4 x MG
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 204.278Å b: 86.338Å c: 114.463Å
α: 90° β: 123.07° γ: 90°
R-values:
R R work R free
0.19 0.188 0.245
Expression system: Escherichia coli BL21(DE3)

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