› 4eal

X-ray diffraction
2.51Å resolution

Co-crystal of AMPK core with ATP soaked with AMP

Released:
DOI: 10.2210/pdb4eal/pdb
PDB entry 4eal coloured by chain, front view.
PDB entry 4eal coloured by chain, side view.
PDB entry 4eal coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
AMP-activated protein kinase undergoes nucleotide-dependent conformational changes.
Chen L, Wang J, Zhang YY, Yan SF, Neumann D, Schlattner U, Wang ZX, Wu JW
Nat Struct Mol Biol 19 716-8 (2012)
PMID: 22659875

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
ATP + [tau protein] = ADP + [tau protein] phosphate
ATP + [hydroxymethylglutaryl-CoA reductase (NADPH)] = ADP + [hydroxymethylglutaryl-CoA reductase (NADPH)] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157065 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
5'-AMP-activated protein kinase catalytic subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 12.1 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54645 (Residues: 405-479, 540-559; Coverage: 17%)
Gene names: Ampk1, Prkaa1
Sequence domains: Adenylate sensor of SNF1-like protein kinase
Structure domains: Kinase associated domain 1, KA1
5'-AMP-activated protein kinase subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 72 amino acids
Theoretical weight: 8.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80386 (Residues: 200-270; Coverage: 26%)
Gene name: Prkab1
Sequence domains: 5'-AMP-activated protein kinase beta subunit, interaction domain
5'-AMP-activated protein kinase subunit gamma-1 Chain: C
Molecule details ›
Chain: C
Length: 330 amino acids
Theoretical weight: 37.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P80385 (Residues: 1-330; Coverage: 100%)
Gene name: Prkag1
Sequence domains: CBS domain

Ligands and Environments

1 bound ligand:
Ligand AMP 2 x AMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: C2
Unit cell:
a: 175.85Å b: 40.503Å c: 77.738Å
α: 90° β: 105.53° γ: 90°
R-values:
R R work R free
0.235 0.232 0.277
Expression system: Escherichia coli

Quick links

Citations

30 review citations

AMPK: Mechanisms of Cellular Energy Sensing and Restoration of Metabolic Balance.
Garcia et al. (2017)
29 more

1 mention without citation

Chromophorylation of cyanobacteriochrome Slr1393 from Synechocystis sp. PCC 6803 is regulated by protein Slr2111 through allosteric interaction.
He et al. (2018)