› 4ech

X-ray diffraction
2.4Å resolution

Yeast Polyamine Oxidase FMS1, H67Q Mutant

Released:
DOI: 10.2210/pdb4ech/pdb
PDB entry 4ech coloured by chain, front view.
PDB entry 4ech coloured by chain, side view.
PDB entry 4ech coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Mechanistic and structural analyses of the role of His67 in the yeast polyamine oxidase Fms1.
Adachi MS, Taylor AB, Hart PJ, Fitzpatrick PF
Biochemistry 51 4888-97 (2012)
PMID: 22642831

Function and Biology Details

Reaction catalysed: 
N(1)-acetylspermidine + O(2) + H(2)O = putrescine + 3-acetamidopropanal + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyamine oxidase FMS1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 516 amino acids
Theoretical weight: 58.93 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P50264 (Residues: 1-508; Coverage: 100%)
Gene names: FMS1, YM9711.09, YMR020W
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: C2
Unit cell:
a: 161.16Å b: 102Å c: 75.83Å
α: 90° β: 93.12° γ: 90°
R-values:
R R work R free
0.218 0.216 0.265
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

A plant spermine oxidase/dehydrogenase regulated by the proteasome and polyamines.
Ahou et al. (2014)
3 more

2 mentions without citation

Mechanistic and structural analyses of the role of His67 in the yeast polyamine oxidase Fms1.
Adachi et al. (2012)
1 more