› 4edo

X-ray diffraction
1.8Å resolution

Crystal structure of far-red fluorescent protein eqFP650

Released:
DOI: 10.2210/pdb4edo/pdb
PDB entry 4edo coloured by chain, front view.
PDB entry 4edo coloured by chain, side view.
PDB entry 4edo coloured by chain, top view.
Source organism: Entacmaea quadricolor
Primary publication:
Structural basis for bathochromic shift of fluorescence in far-red fluorescent proteins eqFP650 and eqFP670.
Pletnev S, Pletneva NV, Souslova EA, Chudakov DM, Lukyanov S, Wlodawer A, Dauter Z, Pletnev V
Acta Crystallogr D Biol Crystallogr 68 1088-97 (2012)
PMID: 22948909

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-125396 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Far-red fluorescent protein eqFP650 Chains: A, B
Molecule details ›
Chains: A, B
Length: 241 amino acids
Theoretical weight: 27.28 KDa
Source organism: Entacmaea quadricolor
Expression system: Escherichia coli
UniProt:
  • Canonical: J9PBR7 (Residues: 1-223; Coverage: 100%)
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand NRQ 2 x NRQ

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P6122
Unit cell:
a: 104.452Å b: 104.452Å c: 214.615Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.189 0.211
Expression system: Escherichia coli

Quick links

Citations

6 citation in other articles

Extended Stokes shift in fluorescent proteins: chromophore-protein interactions in a near-infrared TagRFP675 variant.
Piatkevich et al. (2013)
5 more

3 mentions without citation

Structure-guided wavelength tuning in far-red fluorescent proteins.
Ng et al. (2016)
2 more