› 4ehj

X-ray diffraction
2.71Å resolution

An X-ray Structure of a Putative Phosphogylcerate Kinase from Francisella tularensis subsp. tularensis SCHU S4

Released:
DOI: 10.2210/pdb4ehj/pdb
PDB entry 4ehj coloured by chain, front view.
PDB entry 4ehj coloured by chain, side view.
PDB entry 4ehj coloured by chain, top view.
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Entry authors: Brunzelle JS, Wawrzak Z, Skarina T, Gordon E, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-177659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoglycerate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 392 amino acids
Theoretical weight: 42.27 KDa
Source organism: Francisella tularensis subsp. tularensis SCHU S4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5NF76 (Residues: 1-392; Coverage: 100%)
Gene names: FTT_1367c, pgk
Sequence domains: Phosphoglycerate kinase
Structure domains: Phosphoglycerate kinase, N-terminal domain

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2221
Unit cell:
a: 98.733Å b: 117.185Å c: 143.759Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.218
Expression system: Escherichia coli BL21(DE3)

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