› 4er8

X-ray diffraction
2.6Å resolution

Structure of the REP associates tyrosine transposase bound to a REP hairpin

Released:
DOI: 10.2210/pdb4er8/pdb
PDB entry 4er8 coloured by chain, front view.
PDB entry 4er8 coloured by chain, side view.
PDB entry 4er8 coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
The processing of repetitive extragenic palindromes: the structure of a repetitive extragenic palindrome bound to its associated nuclease.
Messing SA, Ton-Hoang B, Hickman AB, McCubbin AJ, Peaslee GF, Ghirlando R, Chandler M, Dyda F
Nucleic Acids Res 40 9964-79 (2012)
PMID: 22885300

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-117431 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
REP-associated tyrosine transposase Chain: A
Molecule details ›
Chain: A
Length: 165 amino acids
Theoretical weight: 20.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47152 (Residues: 1-165; Coverage: 100%)
Gene names: JW0218, TnpAREP, b0228, rayT, yafM
Structure domains: Transposase IS200-like
DNA (32-MER) Chain: B
Molecule details ›
Chain: B
Length: 32 nucleotides
Theoretical weight: 9.91 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NI 1 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21212
Unit cell:
a: 100.83Å b: 60.3Å c: 70.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.281
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

6 review citations

Bacterial insertion sequences: their genomic impact and diversity.
Siguier et al. (2014)
5 more

6 mentions without citation

Breaking and joining single-stranded DNA: the HUH endonuclease superfamily.
Chandler et al. (2013)
5 more