› 4es9

X-ray diffraction
2Å resolution

Crystal Structure of the adhesin domain of Epf from Streptococcus pyogenes in P21

Released:
DOI: 10.2210/pdb4es9/pdb
PDB entry 4es9 coloured by chain, front view.
PDB entry 4es9 coloured by chain, side view.
PDB entry 4es9 coloured by chain, top view.
Source organism: Streptococcus pyogenes M49 591
Primary publication:
The extracellular protein factor Epf from Streptococcus pyogenes is a cell surface adhesin that binds to cells through an N-terminal domain containing a carbohydrate-binding module.
Linke C, Siemens N, Oehmcke S, Radjainia M, Law RH, Whisstock JC, Baker EN, Kreikemeyer B
J Biol Chem 287 38178-89 (2012)
PMID: 22977243

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gram-positive cocci surface proteins LPxTG domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 320 amino acids
Theoretical weight: 35.8 KDa
Source organism: Streptococcus pyogenes M49 591
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3BY62 (Residues: 20-332; Coverage: 28%)
Gene name: Spy49_0567
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: P21
Unit cell:
a: 71.08Å b: 117.134Å c: 73.036Å
α: 90° β: 106.46° γ: 90°
R-values:
R R work R free
0.174 0.172 0.215
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structural basis of Staphylococcus epidermidis biofilm formation: mechanisms and molecular interactions.
Büttner et al. (2015)
5 more

1 mention without citation

The extracellular protein factor Epf from Streptococcus pyogenes is a cell surface adhesin that binds to cells through an N-terminal domain containing a carbohydrate-binding module.
Linke et al. (2012)