› 4f4f

X-ray diffraction
1.9Å resolution

X-Ray crystal structure of PLP bound Threonine synthase from Brucella melitensis

Released:
DOI: 10.2210/pdb4f4f/pdb
PDB entry 4f4f coloured by chain, front view.
PDB entry 4f4f coloured by chain, side view.
PDB entry 4f4f coloured by chain, top view.
Source organism: Brucella melitensis bv. 1 str. 16M
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed: 
O-phospho-L-homoserine + H(2)O = L-threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187053 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Threonine synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 468 amino acids
Theoretical weight: 50.57 KDa
Source organism: Brucella melitensis bv. 1 str. 16M
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8YFS0 (Residues: 1-463; Coverage: 100%)
Gene name: BMEI1450
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21
Unit cell:
a: 51.99Å b: 100.01Å c: 92.13Å
α: 90° β: 91.29° γ: 90°
R-values:
R R work R free
0.177 0.175 0.206
Expression system: Escherichia coli

Quick links