› 4f55

X-ray diffraction
1.85Å resolution

Crystal Structure of the Catalytic Domain of the Bacillus cereus SleB Protein

Released:
DOI: 10.2210/pdb4f55/pdb
PDB entry 4f55 coloured by chain, front view.
PDB entry 4f55 coloured by chain, side view.
PDB entry 4f55 coloured by chain, top view.
Source organism: Bacillus cereus ATCC 14579
Primary publication:
Crystal structure of the catalytic domain of the Bacillus cereus SleB protein, important in cortex peptidoglycan degradation during spore germination.
Li Y, Jin K, Setlow B, Setlow P, Hao B
J Bacteriol 194 4537-45 (2012)
PMID: 22730118

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141226 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spore cortex-lytic enzyme Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 13.91 KDa
Source organism: Bacillus cereus ATCC 14579
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0A3V0 (Residues: 136-259; Coverage: 55%)
Gene names: BC_2753, sleB
Sequence domains: Cell Wall Hydrolase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: I432
Unit cell:
a: 134.626Å b: 134.626Å c: 134.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.212
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Germination of spores of Bacillus species: what we know and do not know.
Setlow P. (2014)
4 more

7 mentions without citation

Lytic transglycosylases: concinnity in concision of the bacterial cell wall.
Dik et al. (2017)
6 more