› 4f9u

X-ray diffraction
1.8Å resolution

Structure of glycosylated glutaminyl cyclase from Drosophila melanogaster

Released:
DOI: 10.2210/pdb4f9u/pdb
PDB entry 4f9u coloured by chain, front view.
PDB entry 4f9u coloured by chain, side view.
PDB entry 4f9u coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
Crystal structures of glutaminyl cyclases (QCs) from Drosophila melanogaster reveal active site conservation between insect and mammalian QCs.
Koch B, Kolenko P, Buchholz M, Carrillo DR, Parthier C, Wermann M, Rahfeld JU, Reuter G, Schilling S, Stubbs MT, Demuth HU
Biochemistry 51 7383-92 (2012)
PMID: 22897232

Function and Biology Details

Reaction catalysed: 
L-glutaminyl-peptide = 5-oxoprolyl-peptide + NH(3)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194296 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Glutaminyl-peptide cyclotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.34 KDa
Source organism: Drosophila melanogaster
Expression system: Komagataella pastoris
UniProt:
  • Canonical: Q9VRQ9 (Residues: 29-340; Coverage: 98%)
Gene names: CG32412, QC
Sequence domains: Peptidase family M28
Structure domains: Zn peptidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
3 bound ligands:
Ligand ZN 2 x ZN
Ligand PBD 2 x PBD
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 60.665Å b: 56.964Å c: 95.669Å
α: 90° β: 108.16° γ: 90°
R-values:
R R work R free
0.171 0.17 0.225
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Development and evolution of human glutaminyl cyclase inhibitors (QCIs): an alternative promising approach for disease-modifying treatment of Alzheimer's disease.
Chen et al. (2023)
4 other articles

4 mentions without citation

Transamidase subunit GAA1/GPAA1 is a M28 family metallo-peptide-synthetase that catalyzes the peptide bond formation between the substrate protein's omega-site and the GPI lipid anchor's phosphoethanolamine.
Eisenhaber et al. (2014)
3 more