› 4fcm

X-ray diffraction
2.69Å resolution

Crystal structure of the NTF2-like domain of human G3BP1 in complex with a peptide

Released:
DOI: 10.2210/pdb4fcm/pdb
PDB entry 4fcm coloured by chain, front view.
PDB entry 4fcm coloured by chain, side view.
PDB entry 4fcm coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of the human G3BP1 NTF2-like domain visualize FxFG Nup repeat specificity.
Vognsen T, Møller IR, Kristensen O
OpenAccess logo PLoS One 8 e80947 (2013)
PMID: 24324649

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-552931 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras GTPase-activating protein-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 16.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13283 (Residues: 1-139; Coverage: 30%)
Gene names: G3BP, G3BP1
Sequence domains: Nuclear transport factor 2 (NTF2) domain
Structure domains: Nuclear Transport Factor 2; Chain: A,
Nucleoporin repeat peptide Chains: C, D
Molecule details ›
Chains: C, D
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P212121
Unit cell:
a: 42.78Å b: 71.49Å c: 89.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.225 0.282
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Molecular mechanisms of stress granule assembly and disassembly.
Hofmann et al. (2021)
8 more

6 mentions without citation

G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.
Yang et al. (2020)
5 more