› 4fhx

X-ray diffraction
2.7Å resolution

Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity - H336N mutant bound to MgATP

Released:
DOI: 10.2210/pdb4fhx/pdb
PDB entry 4fhx coloured by chain, front view.
PDB entry 4fhx coloured by chain, side view.
PDB entry 4fhx coloured by chain, top view.
Source organism: Schizosaccharomyces pombe 972h-
Primary publication:
Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity.
Lunde BM, Magler I, Meinhart A
OpenAccess logo Nucleic Acids Res 40 9815-24 (2012)
PMID: 22885303

Function and Biology Details

Reactions catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
UTP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Terminal uridylyltransferase cid1 Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 39.93 KDa
Source organism: Schizosaccharomyces pombe 972h-
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O13833 (Residues: 33-377; Coverage: 85%)
Gene names: SPAC19D5.03, cid1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ATP 1 x ATP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 53.72Å b: 62.47Å c: 111.826Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Cytoplasmic RNA: a case of the tail wagging the dog.
Norbury CJ. (2013)
2 more

3 mentions without citation

Crystal structures of the Cid1 poly (U) polymerase reveal the mechanism for UTP selectivity.
Lunde et al. (2012)
2 more