› 4fls

X-ray diffraction
2.3Å resolution

Crystal structure of Amylosucrase inactive double mutant F290K-E328Q from Neisseria polysaccharea in complex with sucrose.

Released:
DOI: 10.2210/pdb4fls/pdb
PDB entry 4fls coloured by chain, front view.
PDB entry 4fls coloured by chain, side view.
PDB entry 4fls coloured by chain, top view.
Source organism: Neisseria polysaccharea
Primary publication:
Applying pairwise combinations of amino acid mutations for sorting out highly efficient glucosylation tools for chemo-enzymatic synthesis of bacterial oligosaccharides.
Champion E, Guérin F, Moulis C, Barbe S, Tran TH, Morel S, Descroix K, Monsan P, Mourey L, Mulard LA, Tranier S, Remaud-Siméon M, André I
J Am Chem Soc 134 18677-88 (2012)
PMID: 23072374

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->4)-alpha-D-glucosyl)(n) = D-fructose + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Amylosucrase Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 71.55 KDa
Source organism: Neisseria polysaccharea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZEU2 (Residues: 13-636; Coverage: 98%)
Gene name: ams
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
2 bound ligands:
Ligand CL 1 x CL
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 95.11Å b: 114.7Å c: 54.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.22
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Strategies for the discovery and engineering of enzymes for biocatalysis.
Davids et al. (2013)
5 more

1 mention without citation

The N253F mutant structure of trehalose synthase from Deinococcus radiodurans reveals an open active-site topology.
Chow et al. (2017)