› 4fpd

X-ray diffraction
2.65Å resolution

Deprotonation of D96 in bacteriorhodopsin opens the proton uptake pathway

Released:
DOI: 10.2210/pdb4fpd/pdb
PDB entry 4fpd coloured by chain, front view.
PDB entry 4fpd coloured by chain, side view.
PDB entry 4fpd coloured by chain, top view.
Source organism: Halobacterium salinarum NRC-1
Primary publication:
Deprotonation of D96 in bacteriorhodopsin opens the proton uptake pathway.
Wang T, Sessions AO, Lunde CS, Rouhani S, Glaeser RM, Duan Y, Facciotti MT
Structure 21 290-7 (2013)
PMID: 23394942

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriorhodopsin Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.13 KDa
Source organism: Halobacterium salinarum NRC-1
Expression system: Escherichia coli
UniProt:
  • Canonical: P02945 (Residues: 1-262; Coverage: 100%)
Gene names: VNG_1467G, bop
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

3 bound ligands:
Ligand RET 1 x RET
Ligand CL 1 x CL
Ligand LI1 8 x LI1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P63
Unit cell:
a: 60.931Å b: 60.931Å c: 107.701Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.237 0.223 0.24
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Bacteriorhodopsin: Would the real structural intermediates please stand up?
Wickstrand et al. (2015)
3 more

4 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
3 more