› 4fqz

X-ray diffraction
2.8Å resolution

Crystal structure of a protease-resistant mutant form of human galectin-8

Released:
DOI: 10.2210/pdb4fqz/pdb
PDB entry 4fqz coloured by chain, front view.
PDB entry 4fqz coloured by chain, side view.
PDB entry 4fqz coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structure of a protease-resistant mutant form of human galectin-8 with two carbohydrate recognition domains.
Yoshida H, Yamashita S, Teraoka M, Itoh A, Nakakita S, Nishi N, Kamitori S
FEBS J 279 3937-51 (2012)
PMID: 22913484

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-517137 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galectin-8 Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.11 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00214 (Residues: 1-155, 184-317; Coverage: 91%)
Gene name: LGALS8
Sequence domains: Galactoside-binding lectin
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
1 bound ligand:
Ligand EDO 10 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P43212
Unit cell:
a: 79.259Å b: 79.259Å c: 129.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.209 0.253
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Galectin-8: a matricellular lectin with key roles in angiogenesis.
Troncoso et al. (2014)
4 more

4 mentions without citation

Sterical hindrance promotes selectivity of the autophagy cargo receptor NDP52 for the danger receptor galectin-8 in antibacterial autophagy.
Li et al. (2013)
3 more