› 4fvu

X-ray diffraction
2.91Å resolution

Structural basis for the dsRNA specificity of the Lassa virus NP exonuclease

Released:
DOI: 10.2210/pdb4fvu/pdb
PDB entry 4fvu coloured by chain, front view.
PDB entry 4fvu coloured by chain, side view.
PDB entry 4fvu coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for the dsRNA specificity of the Lassa virus NP exonuclease.
Hastie KM, King LB, Zandonatti MA, Saphire EO
OpenAccess logo PLoS One 7 e44211 (2012)
PMID: 22937163

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146711 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.21 KDa
Source organism: Lassa virus Josiah
Expression system: Escherichia coli
UniProt:
  • Canonical: P13699 (Residues: 342-569; Coverage: 40%)
Gene name: N
Sequence domains: Arenavirus nucleocapsid C-terminal domain
Structure domains: Arenaviral nucleoprotein, C-terminal domain
RNA (5'-R(*GP*GP*AP*GP*GP*GP*AP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.68 KDa
Source organism: synthetic construct
Expression system: Not provided
RNA (5'-R(*CP*UP*CP*CP*CP*UP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 44.772Å b: 84.311Å c: 79.809Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.254
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

12 review citations

The SARS coronavirus nucleocapsid protein--forms and functions.
Chang et al. (2014)
11 more

10 mentions without citation

Remdesivir and SARS-CoV-2: Structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites.
Shannon et al. (2020)
9 more