› 4g0m

X-ray diffraction
2.31Å resolution

Crystal structure of Arabidopsis thaliana AGO2 MID domain

Released:
DOI: 10.2210/pdb4g0m/pdb
PDB entry 4g0m coloured by chain, front view.
PDB entry 4g0m coloured by chain, side view.
PDB entry 4g0m coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Frank F, Hauver J, Sonenberg N, Nagar B
EMBO J 31 3588-95 (2012)
PMID: 22850669

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193459 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 150 amino acids
Theoretical weight: 16.68 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SHF3 (Residues: 579-727; Coverage: 15%)
Gene names: AGO2, At1g31280, T19E23.7
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P43212
Unit cell:
a: 67.647Å b: 67.647Å c: 223.222Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.206 0.237
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Argonaute proteins: functional insights and emerging roles.
Meister G. (2013)
17 more

1 mention without citation

Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Frank et al. (2012)