› 4g0o

X-ray diffraction
2.19Å resolution

Crystal structure of Arabidopsis thaliana AGO5 MID domain

Released:
DOI: 10.2210/pdb4g0o/pdb
PDB entry 4g0o coloured by chain, front view.
PDB entry 4g0o coloured by chain, side view.
PDB entry 4g0o coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Frank F, Hauver J, Sonenberg N, Nagar B
EMBO J 31 3588-95 (2012)
PMID: 22850669

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193468 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute 5 Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.48 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9SJK3 (Residues: 562-699; Coverage: 14%)
Gene names: AGO5, At2g27880, T1E2.20
Sequence domains: Mid domain of argonaute
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 34.823Å b: 37.078Å c: 62.733Å
α: 75.4° β: 76.06° γ: 90.12°
R-values:
R R work R free
0.22 0.217 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Argonaute proteins: functional insights and emerging roles.
Meister G. (2013)
17 more

1 mention without citation

Arabidopsis Argonaute MID domains use their nucleotide specificity loop to sort small RNAs.
Frank et al. (2012)