› 4g7h

X-ray diffraction
2.9Å resolution

Crystal structure of Thermus thermophilus transcription initiation complex

Released:
DOI: 10.2210/pdb4g7h/pdb
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PDB entry 4g7h coloured by chain, front view.
PDB entry 4g7h coloured by chain, side view.
PDB entry 4g7h coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Structural basis of transcription initiation.
Zhang Y, Feng Y, Chatterjee S, Tuske S, Ho MX, Arnold E, Ebright RH
Science 338 1076-80 (2012)
PMID: 23086998

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-117531 (preferred)
Entry contents:
5 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (7 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, K, L
Molecule details ›
Chains: A, B, K, L
Length: 315 amino acids
Theoretical weight: 35.06 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q5SHR6 (Residues: 1-315; Coverage: 100%)
Gene names: TTHA1664, rpoA
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta Chains: C, M
Molecule details ›
Chains: C, M
Length: 1119 amino acids
Theoretical weight: 125.44 KDa
Source organism: Thermus thermophilus HB8
UniProt:
  • Canonical: Q8RQE9 (Residues: 1-1119; Coverage: 100%)
Gene names: TTHA1813, rpoB
Sequence domains:
Structure domains:
DNA-directed RNA polymerase subunit beta' Chains: D, N
DNA-directed RNA polymerase subunit omega Chains: E, O
RNA polymerase sigma factor SigA Chains: F, P
5'-D(*CP*CP*T*GP*CP*AP*TP*CP*CP*GP*TP*GP*AP*GP*TP*CP*GP*AP*G)-3' Chains: G, Q
5'-D(*TP*AP*TP*AP*AP*TP*GP*GP*GP*AP*GP*CP*TP*GP*TP*CP*AP*CP*GP*GP*AP*TP*GP*CP*AP*GP*G)-3' Chains: H, R

Ligands and Environments

2 bound ligands:
Ligand MG 10 x MG
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 185.56Å b: 104.57Å c: 297.55Å
α: 90° β: 98.32° γ: 90°
R-values:
R R work R free
0.188 0.188 0.226
Expression system: Escherichia coli BL21(DE3)

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Citations

45 review citations

Bacterial sigma factors: a historical, structural, and genomic perspective.
Feklístov et al. (2014)
44 more

24 mentions without citation

Recent functional insights into the role of (p)ppGpp in bacterial physiology.
Hauryliuk et al. (2015)
23 more

PDB-REDO

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