› 4g80

X-ray diffraction
3.58Å resolution

Crystal structure of voltage sensing domain of Ci-VSP with fragment antibody (WT, 3.8 A)

Released:
DOI: 10.2210/pdb4g80/pdb
PDB entry 4g80 coloured by chain, front view.
PDB entry 4g80 coloured by chain, side view.
PDB entry 4g80 coloured by chain, top view.
Source organisms:
Primary publication:
Structural mechanism of voltage-dependent gating in an isolated voltage-sensing domain.
Li Q, Wanderling S, Paduch M, Medovoy D, Singharoy A, McGreevy R, Villalba-Galea CA, Hulse RE, Roux B, Schulten K, Kossiakoff A, Perozo E
Nat Struct Mol Biol 21 244-52 (2014)
PMID: 24487958

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207863 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase Chains: I, J, S, T
Molecule details ›
Chains: I, J, S, T
Length: 155 amino acids
Theoretical weight: 17.75 KDa
Source organism: Ciona intestinalis
Expression system: Escherichia coli
UniProt:
  • Canonical: F6XHE4 (Residues: 100-238; Coverage: 24%)
Sequence domains: Ion transport protein
Structure domains: Voltage-gated potassium channels. Chain C
fragment antibody heavy chain Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 224 amino acids
Theoretical weight: 23.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins
fragment antibody light chain Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 211 amino acids
Theoretical weight: 22.99 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B, APS BEAMLINE 23-ID-D, APS BEAMLINE 24-ID-C, APS BEAMLINE 24-ID-E
Spacegroup: P1
Unit cell:
a: 77.29Å b: 94.24Å c: 193.95Å
α: 102.63° β: 93.45° γ: 105.25°
R-values:
R R work R free
0.25 0.248 0.292
Expression system: Escherichia coli

Quick links

Citations

43 review citations

Molecular Dynamics Simulation for All.
Hollingsworth et al. (2018)
42 more

22 mentions without citation

Voltage-Gated Potassium Channels: A Structural Examination of Selectivity and Gating.
Kim et al. (2016)
21 more