› 4g8s

X-ray diffraction
2.2Å resolution

Crystal Structure Of a Putative Nitroreductase from Geobacter sulfurreducens PCA (Target PSI-013445)

Released:
DOI: 10.2210/pdb4g8s/pdb
PDB entry 4g8s coloured by chain, front view.
PDB entry 4g8s coloured by chain, side view.
PDB entry 4g8s coloured by chain, top view.
Source organism: Geobacter sulfurreducens PCA
Entry authors: Kumar PR, Bhosle R, Hillerich B, Seidel R, Toro R, Almo SC, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181070 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitroreductase domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.43 KDa
Source organism: Geobacter sulfurreducens PCA
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q74GM9 (Residues: 1-188; Coverage: 100%)
Gene name: GSU0217
Sequence domains: Nitroreductase family
Structure domains: NADH Oxidase

Ligands and Environments

2 bound ligands:
Ligand FMN 2 x FMN
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P43212
Unit cell:
a: 73.759Å b: 73.759Å c: 169.472Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.162 0.197
Expression system: Escherichia coli BL21(DE3)

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