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SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid.

Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA
Proc Natl Acad Sci U S A 110 10586-91 (2013)
Related entries: 4gck, 4gct, 4gfk, 4gfl

Cited: 56 times
EuropePMC logo PMID: 23754405

Abstract

The spatial and temporal control of Filamenting temperature sensitive mutant Z (FtsZ) Z-ring formation is crucial for proper cell division in bacteria. In Escherichia coli, the synthetic lethal with a defective Min system (SlmA) protein helps mediate nucleoid occlusion, which prevents chromosome fragmentation by binding FtsZ and inhibiting Z-ring formation over the nucleoid. However, to perform its function, SlmA must be bound to the nucleoid. To deduce the basis for this chromosomal requirement, we performed biochemical, cellular, and structural studies. Strikingly, structures show that SlmA dramatically distorts DNA, allowing it to bind as an orientated dimer-of-dimers. Biochemical data indicate that SlmA dimer-of-dimers can spread along the DNA. Combined structural and biochemical data suggest that this DNA-activated SlmA oligomerization would prevent FtsZ protofilament propagation and bundling. Bioinformatic analyses localize SlmA DNA sites near membrane-tethered chromosomal regions, and cellular studies show that SlmA inhibits FtsZ reservoirs from forming membrane-tethered Z rings. Thus, our combined data indicate that SlmA DNA helps block Z-ring formation over chromosomal DNA by forming higher-order protein-nucleic acid complexes that disable FtsZ filaments from coalescing into proper structures needed for Z-ring creation.

Articles - 4gcl mentioned but not cited (3)

  1. SlmA forms a higher-order structure on DNA that inhibits cytokinetic Z-ring formation over the nucleoid. Tonthat NK, Milam SL, Chinnam N, Whitfill T, Margolin W, Schumacher MA. Proc Natl Acad Sci U S A 110 10586-10591 (2013)
  2. SlmA antagonism of FtsZ assembly employs a two-pronged mechanism like MinCD. Du S, Lutkenhaus J. PLoS Genet 10 e1004460 (2014)
  3. Structures of the nucleoid occlusion protein SlmA bound to DNA and the C-terminal domain of the cytoskeletal protein FtsZ. Schumacher MA, Zeng W. Proc Natl Acad Sci U S A 113 4988-4993 (2016)


Reviews citing this publication (17)

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Articles citing this publication (36)

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  21. The Bacterial DNA Binding Protein MatP Involved in Linking the Nucleoid Terminal Domain to the Divisome at Midcell Interacts with Lipid Membranes. Monterroso B, Zorrilla S, Sobrinos-Sanguino M, Robles-Ramos MÁ, Alfonso C, Söderström B, Meiresonne NY, Verheul J, den Blaauwen T, Rivas G. mBio 10 e00376-19 (2019)
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