› 4ges

X-ray diffraction
1.23Å resolution

crystal structure of GFP-TYR151PYZ with an unnatural amino acid incorporation

Released:
DOI: 10.2210/pdb4ges/pdb
Model geometry
Fit model/data
PDB entry 4ges coloured by chain, front view.
PDB entry 4ges coloured by chain, side view.
PDB entry 4ges coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Genetic incorporation of a metal-chelating amino acid as a probe for protein electron transfer.
Liu X, Li J, Dong J, Hu C, Gong W, Wang J
Angew Chem Int Ed Engl 51 10261-5 (2012)
PMID: 22936654

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: B
Molecule details ›
Chain: B
Length: 246 amino acids
Theoretical weight: 27.88 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 0WZ 1 x 0WZ

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 67.717Å b: 50.809Å c: 62.364Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.163 0.188
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Playing with the Molecules of Life.
Young et al. (2018)
5 more

1 mention without citation

PAU-1, a Novel Plasmid-Encoded Ambler Class A β-Lactamase Identified in a Clinical Pseudomonas aeruginosa Isolate.
Wang et al. (2019)