› 4ggm

X-ray diffraction
2.9Å resolution

Structure of LpxI

Released:
DOI: 10.2210/pdb4ggm/pdb
PDB entry 4ggm coloured by chain, front view.
PDB entry 4ggm coloured by chain, side view.
PDB entry 4ggm coloured by chain, top view.
Source organism: Caulobacter vibrioides NA1000
Primary publication:
LpxI structures reveal how a lipid A precursor is synthesized.
Metzger LE, Lee JK, Finer-Moore JS, Raetz CR, Stroud RM
Nat Struct Mol Biol 19 1132-8 (2012)
PMID: 23042606

Function and Biology Details

Reaction catalysed: 
A UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + H(2)O = a lipid X + UMP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101818 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-2,3-diacylglucosamine pyrophosphatase LpxI Chain: X
Molecule details ›
Chain: X
Length: 283 amino acids
Theoretical weight: 30.38 KDa
Source organism: Caulobacter vibrioides NA1000
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0H3C8Q1 (Residues: 1-280; Coverage: 100%)
Gene names: CCNA_01987, lpxI
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand LP5 1 x LP5
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: I4122
Unit cell:
a: 74.286Å b: 74.286Å c: 364.644Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.216 0.272
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Biosynthesis and export of bacterial lipopolysaccharides.
Whitfield et al. (2014)
3 more

5 mentions without citation

Structure, inhibition, and regulation of essential lipid A enzymes.
Zhou et al. (2017)
4 more