› 4gh9

X-ray diffraction
1.65Å resolution

Crystal structure of Marburg virus VP35 RNA binding domain

Released:
DOI: 10.2210/pdb4gh9/pdb
PDB entry 4gh9 coloured by chain, front view.
PDB entry 4gh9 coloured by chain, side view.
PDB entry 4gh9 coloured by chain, top view.
Source organism: Marburg virus - Musoke, Kenya, 1980
Primary publication:
Marburg virus VP35 can both fully coat the backbone and cap the ends of dsRNA for interferon antagonism.
Bale S, Julien JP, Bornholdt ZA, Kimberlin CR, Halfmann P, Zandonatti MA, Kunert J, Kroon GJ, Kawaoka Y, MacRae IJ, Wilson IA, Saphire EO
OpenAccess logo PLoS Pathog 8 e1002916 (2012)
PMID: 23028316

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152873 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polymerase cofactor VP35 Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.3 KDa
Source organism: Marburg virus - Musoke, Kenya, 1980
Expression system: Escherichia coli
UniProt:
  • Canonical: P35259 (Residues: 204-329; Coverage: 38%)
Gene name: VP35
Sequence domains: Filoviridae VP35
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ACT 6 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: C2221
Unit cell:
a: 42.524Å b: 90.88Å c: 65.925Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.161 0.217
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Filovirus pathogenesis and immune evasion: insights from Ebola virus and Marburg virus.
Messaoudi et al. (2015)
20 more

2 mentions without citation

Marburg virus VP35 can both fully coat the backbone and cap the ends of dsRNA for interferon antagonism.
Bale et al. (2012)
1 more