› 4grj

X-ray diffraction
1.15Å resolution

Crystal structure of nitrophorin 4 triple mutant complex with NO

Released:
DOI: 10.2210/pdb4grj/pdb
PDB entry 4grj coloured by chain, front view.
PDB entry 4grj coloured by chain, side view.
PDB entry 4grj coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry authors: Weichsel A, Issaian AV, Montfort WR

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 184 amino acids
Theoretical weight: 20.29 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
2 bound ligands:
Ligand NO 2 x NO
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P3121
Unit cell:
a: 69.64Å b: 69.64Å c: 141.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.16 0.185
Expression system: Escherichia coli

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