› 4gsn

X-ray diffraction
2.3Å resolution

Crystal Structure of GSTe2 ZAN/U variant from Anopheles gambiae

Released:
DOI: 10.2210/pdb4gsn/pdb
PDB entry 4gsn coloured by chain, front view.
PDB entry 4gsn coloured by chain, side view.
PDB entry 4gsn coloured by chain, top view.
Source organism: Anopheles gambiae
Primary publication:
Metabolic and target-site mechanisms combine to confer strong DDT resistance in Anopheles gambiae.
Mitchell SN, Rigden DJ, Dowd AJ, Lu F, Wilding CS, Weetman D, Dadzie S, Jenkins AM, Regna K, Boko P, Djogbenou L, Muskavitch MA, Ranson H, Paine MJ, Mayans O, Donnelly MJ
OpenAccess logo PLoS One 9 e92662 (2014)
PMID: 24675797

Function and Biology Details

Reaction catalysed: 
RX + glutathione = HX + R-S-glutathione
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190411 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
glutathione transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 220 amino acids
Theoretical weight: 24.75 KDa
Source organism: Anopheles gambiae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9GPL8 (Residues: 2-221; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand GSH 4 x GSH
2 bound ligands:
Ligand 1PE 1 x 1PE
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 51.33Å b: 86.38Å c: 92.85Å
α: 90° β: 90.73° γ: 90°
R-values:
R R work R free
0.175 0.173 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

A bibliometric analysis of literature on malaria vector resistance: (1996 - 2015).
Sweileh et al. (2016)
3 more

2 mentions without citation

Metabolic and target-site mechanisms combine to confer strong DDT resistance in Anopheles gambiae.
Mitchell et al. (2014)
1 more