› 4gt3

X-ray diffraction
1.68Å resolution

ATP-bound form of the ERK2 kinase

Released:
DOI: 10.2210/pdb4gt3/pdb
PDB entry 4gt3 coloured by chain, front view.
PDB entry 4gt3 coloured by chain, side view.
PDB entry 4gt3 coloured by chain, top view.
Source organism: Rattus norvegicus
Entry authors: Pozharski E, Zhang J, Shapiro P

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158941 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mitogen-activated protein kinase 1 Chain: A
Molecule details ›
Chain: A
Length: 364 amino acids
Theoretical weight: 42.24 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63086 (Residues: 1-358; Coverage: 100%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand GOL 3 x GOL
Ligand ATP 1 x ATP
Ligand MG 2 x MG
Ligand PEG 1 x PEG
1 modified residue:
Ligand CME 1 x CME

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P21
Unit cell:
a: 48.629Å b: 69.259Å c: 59.816Å
α: 90° β: 109.03° γ: 90°
R-values:
R R work R free
0.168 0.166 0.2
Expression system: Escherichia coli

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