› 4gtu

X-ray diffraction
3.3Å resolution

LIGAND-FREE HOMODIMERIC HUMAN GLUTATHIONE S-TRANSFERASE M4-4

Released:
DOI: 10.2210/pdb4gtu/pdb
PDB entry 4gtu coloured by chain, front view.
PDB entry 4gtu coloured by chain, side view.
PDB entry 4gtu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
An asparagine-phenylalanine substitution accounts for catalytic differences between hGSTM3-3 and other human class mu glutathione S-transferases.
Patskovsky YV, Patskovska LN, Listowsky I
Biochemistry 38 16187-94 (1999)
PMID: 10587441

Function and Biology Details

Reactions catalysed: 
RX + glutathione = HX + R-S-glutathione
Leukotriene C(4) = leukotriene A(4) + glutathione
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169812 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutathione S-transferase Mu 4 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 217 amino acids
Theoretical weight: 25.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q03013 (Residues: 2-218; Coverage: 100%)
Gene name: GSTM4
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 51Å b: 215.7Å c: 94.56Å
α: 90° β: 97.4° γ: 90°
R-values:
R R work R free
0.245 0.245 0.315
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Glutathione transferases.
Hayes et al. (2005)
1 more

5 mentions without citation

Computational interaction analysis of organophosphorus pesticides with different metabolic proteins in humans.
Sharma et al. (2011)
4 more