› 4gv6

X-ray diffraction
1.98Å resolution

Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production

Released:
DOI: 10.2210/pdb4gv6/pdb
PDB entry 4gv6 coloured by chain, front view.
PDB entry 4gv6 coloured by chain, side view.
PDB entry 4gv6 coloured by chain, top view.
Source organism: Lassa virus Josiah
Primary publication:
Structures of arenaviral nucleoproteins with triphosphate dsRNA reveal a unique mechanism of immune suppression.
Jiang X, Huang Q, Wang W, Dong H, Ly H, Liang Y, Dong C
J Biol Chem 288 16949-16959 (2013)
PMID: 23615902

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146714 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 214 amino acids
Theoretical weight: 24.07 KDa
Source organism: Lassa virus Josiah
Expression system: Escherichia coli
UniProt:
  • Canonical: P13699 (Residues: 364-569; Coverage: 36%)
Gene name: N
Sequence domains: Arenavirus nucleocapsid C-terminal domain
Structure domains: Arenaviral nucleoprotein, C-terminal domain
RNA (5'-R(*(GTP)P*GP*GP*C)-3') Chain: B
Molecule details ›
Chain: B
Length: 4 nucleotides
Theoretical weight: 1.46 KDa
Source organism: Lassa virus Josiah
Expression system: Not provided
RNA (5'-R(P*CP*GP*CP*CP*C)-3') Chain: C
Molecule details ›
Chain: C
Length: 5 nucleotides
Theoretical weight: 1.52 KDa
Source organism: Lassa virus Josiah
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand MN 1 x MN
Ligand ZN 1 x ZN
1 modified residue:
Ligand GTP 1 x GTP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 46.51Å b: 46.51Å c: 208.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.229
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

26 review citations

Pattern Recognition and Signaling Mechanisms of RIG-I and MDA5.
Reikine et al. (2014)
25 more

2 mentions without citation

Structures of arenaviral nucleoproteins with triphosphate dsRNA reveal a unique mechanism of immune suppression.
Jiang et al. (2013)
1 more