4gv9

X-ray diffraction
2.46Å resolution

Lassa nucleoprotein C-terminal domain in complex with triphosphated dsRNA soaking for 5 min

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146715 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Nucleoprotein Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 24.01 KDa
Source organism: Lassa virus Josiah
Expression system: Escherichia coli
UniProt:
  • Canonical: P13699 (Residues: 364-569; Coverage: 36%)
Gene name: N
Sequence domains: Arenavirus nucleocapsid C-terminal domain
Structure domains: Arenaviral nucleoprotein, C-terminal domain
RNA (5'-R(*(GTP)P*GP*GP*C)-3') Chain: E
Molecule details ›
Chain: E
Length: 4 nucleotides
Theoretical weight: 1.46 KDa
Source organism: Lassa virus Josiah
Expression system: Not provided
RNA (5'-R(P*CP*GP*CP*CP*C)-3') Chain: F
Molecule details ›
Chain: F
Length: 5 nucleotides
Theoretical weight: 1.52 KDa
Source organism: Lassa virus Josiah
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P41212
Unit cell:
a: 44.78Å b: 44.78Å c: 221.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.259 0.305
Expression systems:
  • Escherichia coli
  • Not provided