› 4gw1

X-ray diffraction
2.24Å resolution

cQFD Meditope

Released:
DOI: 10.2210/pdb4gw1/pdb
PDB entry 4gw1 coloured by chain, front view.
PDB entry 4gw1 coloured by chain, side view.
PDB entry 4gw1 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Identification and grafting of a unique peptide-binding site in the Fab framework of monoclonal antibodies.
Donaldson JM, Zer C, Avery KN, Bzymek KP, Horne DA, Williams JC
Proc Natl Acad Sci U S A 110 17456-61 (2013)
PMID: 24101516

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-211650 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Fab light chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 214 amino acids
Theoretical weight: 23.45 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
Fab heavy chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 221 amino acids
Theoretical weight: 23.73 KDa
Source organism: Mus musculus
Expression system: Not provided
Structure domains: Immunoglobulins
cQFD meditope Chains: E, F
Molecule details ›
Chains: E, F
Length: 12 amino acids
Theoretical weight: 1.47 KDa

Ligands and Environments

1 bound ligand:
Ligand PO4 3 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P212121
Unit cell:
a: 64.257Å b: 82.582Å c: 211.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.226
Expression system: Not provided

Quick links

Citations

3 review citations

Site-specific antibody-drug conjugates: the nexus of bioorthogonal chemistry, protein engineering, and drug development.
Agarwal et al. (2015)
2 more

7 mentions without citation

Molecular evolution of peptides by yeast surface display technology.
Linciano et al. (2019)
6 more