› 4gxf

X-ray diffraction
2.73Å resolution

Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor

Released:
DOI: 10.2210/pdb4gxf/pdb
PDB entry 4gxf coloured by chain, front view.
PDB entry 4gxf coloured by chain, side view.
PDB entry 4gxf coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Involvement of Tyr108 in the enzyme mechanism of the small laccase from Streptomyces coelicolor.
Gupta A, Nederlof I, Sottini S, Tepper AW, Groenen EJ, Thomassen EA, Canters GW
J Am Chem Soc 134 18213-6 (2012)
PMID: 23094962

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194974 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Copper oxidase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 279 amino acids
Theoretical weight: 30.66 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XAL8 (Residues: 38-316; Coverage: 81%)
Gene names: SC4C6.22, SCO6712
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

3 bound ligands:
Ligand CU 12 x CU
Ligand PG4 10 x PG4
Ligand O 3 x O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P43212
Unit cell:
a: 176.66Å b: 176.66Å c: 176.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.199
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Laccase engineering: from rational design to directed evolution.
Mate et al. (2015)
8 more