› 4gxu

X-ray diffraction
3.29Å resolution

Crystal structure of antibody 1F1 bound to the 1918 influenza hemagglutinin

Released:
DOI: 10.2210/pdb4gxu/pdb
PDB entry 4gxu coloured by chain, front view.
PDB entry 4gxu coloured by chain, side view.
PDB entry 4gxu coloured by chain, top view.
Source organisms:
Primary publication:
Influenza human monoclonal antibody 1F1 interacts with three major antigenic sites and residues mediating human receptor specificity in H1N1 viruses.
Tsibane T, Ekiert DC, Krause JC, Martinez O, Crowe JE, Wilson IA, Basler CF
OpenAccess logo PLoS Pathog 8 e1003067 (2012)
PMID: 23236279

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212854 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chains: A, C, E, G, I, K
Molecule details ›
Chains: A, C, E, G, I, K
Length: 331 amino acids
Theoretical weight: 36.45 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9WFX3 (Residues: 18-344; Coverage: 60%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains:
Hemagglutinin HA2 chain Chains: B, D, F, H, J, L
Molecule details ›
Chains: B, D, F, H, J, L
Length: 176 amino acids
Theoretical weight: 20.09 KDa
Source organism: Influenza A virus (A/Brevig Mission/1/1918(H1N1))
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9WFX3 (Residues: 345-520; Coverage: 32%)
Gene name: HA
Sequence domains: Haemagglutinin
Structure domains: Hemagglutinin Ectodomain; Chain B
Antibody 1F1, heavy chain Chains: M, O, Q, S, U, W
Molecule details ›
Chains: M, O, Q, S, U, W
Length: 231 amino acids
Theoretical weight: 24.91 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
Antibody 1F1, light chain Chains: N, P, R, T, V, X
Molecule details ›
Chains: N, P, R, T, V, X
Length: 217 amino acids
Theoretical weight: 22.79 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 12 x NAG
1 modified residue:
Ligand PCA 12 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 153.711Å b: 176.269Å c: 168.12Å
α: 90° β: 92.14° γ: 90°
R-values:
R R work R free
0.221 0.219 0.255
Expression systems:
  • Trichoplusia ni
  • Homo sapiens

Quick links

Citations

16 review citations

From Original Antigenic Sin to the Universal Influenza Virus Vaccine.
Henry et al. (2018)
15 more

15 mentions without citation

A Perspective on the Structural and Functional Constraints for Immune Evasion: Insights from Influenza Virus.
Wu et al. (2017)
14 more