› 4gyc

X-ray diffraction
2.05Å resolution

Structure of the SRII(D75N mutant)/HtrII Complex in I212121 space group ("U" shape)

Released:
DOI: 10.2210/pdb4gyc/pdb
PDB entry 4gyc coloured by chain, front view.
PDB entry 4gyc coloured by chain, side view.
PDB entry 4gyc coloured by chain, top view.
Source organism: Natronomonas pharaonis
Primary publication:
Ground state structure of D75N mutant of sensory rhodopsin II in complex with its cognate transducer.
Ishchenko A, Round E, Borshchevskiy V, Grudinin S, Gushchin I, Klare JP, Balandin T, Remeeva A, Engelhard M, Büldt G, Gordeliy V
J Photochem Photobiol B 123 55-8 (2013)
PMID: 23619282

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154503 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Sensory rhodopsin-2 Chain: A
Molecule details ›
Chain: A
Length: 245 amino acids
Theoretical weight: 26.2 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42196 (Residues: 1-239; Coverage: 100%)
Gene names: sop2, sopII
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins
Sensory rhodopsin II transducer Chain: B
Molecule details ›
Chain: B
Length: 135 amino acids
Theoretical weight: 13.93 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42259 (Residues: 1-135; Coverage: 25%)
Gene names: htr2, htrII
Sequence domains:
Structure domains: Helix hairpin bin

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand LFA 9 x LFA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: I212121
Unit cell:
a: 49.465Å b: 113.674Å c: 126.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.203 0.223
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Transmembrane Signal Transduction in Two-Component Systems: Piston, Scissoring, or Helical Rotation?
Gushchin et al. (2018)
3 other articles

2 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
1 more