› 4h14

X-ray diffraction
1.55Å resolution

Crystal Structure of Bovine Coronavirus Spike Protein Lectin Domain

Released:
DOI: 10.2210/pdb4h14/pdb
PDB entry 4h14 coloured by chain, front view.
PDB entry 4h14 coloured by chain, side view.
PDB entry 4h14 coloured by chain, top view.
Source organism: Bovine coronavirus
Primary publication:
Crystal structure of bovine coronavirus spike protein lectin domain.
Peng G, Xu L, Lin YL, Chen L, Pasquarella JR, Holmes KV, Li F
J Biol Chem 287 41931-8 (2012)
PMID: 23091051

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172880 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Spike glycoprotein Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.01 KDa
Source organism: Bovine coronavirus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q1HLC5 (Residues: 7-290; Coverage: 32%)
Sequence domains: Betacoronavirus-like spike glycoprotein S1, N-terminal
Structure domains: Spike glycoprotein, N-terminal domain

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P3121
Unit cell:
a: 86.563Å b: 86.563Å c: 78.068Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.163 0.163 0.177
Expression system: Spodoptera frugiperda

Quick links

Citations

26 review citations

Coronavirus Spike Protein and Tropism Changes.
Hulswit et al. (2016)
25 more

20 mentions without citation

Receptor recognition mechanisms of coronaviruses: a decade of structural studies.
Li F. (2015)
19 more